Composite

Part:BBa_K1055007:Design

Designed by: Sven Jager, Sebastian Wagner and Anne Schieferdecker   Group: iGEM13_TU_Darmstadt   (2013-09-18)


FsC: functional cutinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NotI site found at 587
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 733
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal AgeI site found at 1420
    Illegal AgeI site found at 1532
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 509


Design Notes

Despite BioBricks the part also consists of the RBS from pET22b(+), a TEV-site and a His-Tag.


Source

fungus Fusarium solani pisi

References